Chloride Assay Kit (Colorimetric) (BA0033)

Product Type:
Detection Method:
Microplate Reader
Sample Type:
Biological, Food, Environment
Research Area:
Food Safety & Analysis
Plant & Environmental Stress
Clinical Chemistry
Frequently bought together:


ELISA Kit Technical ManualMSDS

Chloride Assay Kit - Information

Assay Genie's chloride assay kit is designed to measure chloride directly in biological samples without any pretreatment. This Chloride Assay kit based on the improved Fried method utilizes mercuric 2,4,6-tripyridyl-s-triazine, which forms a colored complex specifically with chloride. The intensity of the color for this chloride assay is measured at 610nm. This is directly proportional to the chloride concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.


For quantitative determination of chloride ion and evaluation of drug effects on chloride metabolism.

Chloride Assay Kit - Key Features

  • Sensitive and accurate. Use as little as 5 samples. Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) Cl- in 96-well plate assay.
  • Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
  • Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
  • Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc.

Chloride Assay Kit - Data Sheet

Kit IncludesReagent: 50 mL Chloride standard: 1 mL 35 mg/dL Cl-
Kit RequiresPipeting devices and accessories, clear flat-bottom 96-well plates and plate reader, or spectrophotometer and cuvets.
Method of DetectionOD610nm
Detection Limit0.7 mg/dL (0.2 mM)
SamplesBiological, food and environment
Protocol Length5 min
Size250 tests
Shelf Life12 months
StorageStore Reagent and Standard at 4°C

More Details

Chloride is the major extracellular anion in human body fluids. Chloride plays a key role in maintaining proper water distribution, osmotic pressure and electrolyte balance. Low chloride concentrations may be found with prolonged vomiting, extensive burns, metabolic acidosis, Addisonia crisis and renal diseases. Elevated chloride concentrations are associated with dehydration, congestive heart failure, hyperventilation and urinary obstructions. Determination of chloride in sweat is useful in diagnosing cystic fibrosis. Simple, direct and automation-ready procedures for measuring chloride concentration in biological samples are becoming popular in Research and Drug Discovery.