The CHM Antibody (CAB8345) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-raised antibody is highly specific to human samples and is suitable for use in Western blot applications. By binding to CHM, this antibody enables the detection and analysis of CHM expression in various cell types, making it ideal for studies in cell biology and molecular biology research.CHM, also known as Rab escort protein 1, plays a crucial role in the trafficking and localization of Rab GTPases, which are essential for vesicular transport within cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CHM Antibody
SKU:
CAB8345
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TCD, GGTA, REP-1, DXS540, HSD-32, CHM
Positive Sample:
U-251MG, LO2, NCI-H460, HeLa
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
73kDa
Observed MW:
101kDa
This gene encodes component A of the RAB geranylgeranyl transferase holoenzyme. In the dimeric holoenzyme, this subunit binds unprenylated Rab GTPases and then presents them to the catalytic Rab GGTase subunit for the geranylgeranyl transfer reaction. Rab GTPases need to be geranylgeranyled on either one or two cysteine residues in their C-terminus to localize to the correct intracellular membrane. Mutations in this gene are a cause of choroideremia; also known as tapetochoroidal dystrophy (TCD). This X-linked disease is characterized by progressive dystrophy of the choroid, retinal pigment epithelium and retina. Alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
1121
RRID
AB_2768899
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CHM Rabbit pAb (CAB8345) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 5s.
