The CHMP2A Antibody (CAB10380) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the CHMP2A protein, this antibody enables precise detection and analysis in various cell types, making it an essential component for studies in cell biology and molecular biology.CHMP2A is a key player in the ESCRT pathway, which plays a crucial role in endosomal trafficking, membrane budding, and cytokinesis. Dysregulation of this pathway has been implicated in various diseases, including cancer, neurodegenerative disorders, and viral infections.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CHMP2A Antibody
SKU:
CAB10380
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BC2, BC-2, VPS2, CHMP2, VPS2A, CHMP2A
Positive Sample:
A-431, HepG2, SW480
Cellular Localization:
Cytoplasmic Side, Late Endosome Membrane, Peripheral Membrane Protein.
Calculated MW:
25kDa
Observed MW:
37kDa
CHMP2A belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).
Purification Method
Affinity purification
Gene ID
27243
RRID
AB_2757928
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CHMP2A Rabbit pAb (CAB10380) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
