The CHMP6 Antibody (CAB4975) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.CHMP6 is involved in the formation of multivesicular bodies (MVBs) and the sorting of endocytic cargo for degradation or recycling. Dysregulation of the ESCRT machinery, including CHMP6, has been linked to various diseases, making it a target of interest in studies on cancer, viral infections, and neurodegenerative disorders.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CHMP6 Antibody
SKU:
CAB4975
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
LNKM HQVM SIEE VERI LDET QEAV EYQR QIDE LLAG SFTQ EDED AILE ELSA ITQE QIEL PEVP SEPL PEKI PENV PVKA RPRQ AELV AA
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VPS20, CHMP6
Positive Sample:
U2OS, PC-3, Jurkat
Cellular Localization:
Endomembrane System, Endosome Membrane, Late Endosome Membrane, Lipid-Anchor, Membrane.
Calculated MW:
23kDa
Observed MW:
30kDa
This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein family. Proteins in this family are part of the ESCRT-III (endosomal sorting complex required for transport III) which degrades surface receptors, and in biosynthesis of endosomes.
Purification Method
Affinity purification
Gene ID
79643
RRID
AB_2765965
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CHMP6 Rabbit pAb (CAB4975) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of NIH/3T3 cells using CHMP6 Rabbit pAb (CAB4975) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
