The CHMP7 Antibody (CAB14360) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for CHMP7 in human samples and is validated for use in Western blot applications. By targeting the CHMP7 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for research in cell biology and related fields.CHMP7 is a key player in the endosomal sorting pathway, playing a role in the formation of multivesicular bodies and cellular cargo sorting.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CHMP7 Antibody
SKU:
CAB14360
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CHMP7
Positive Sample:
HeLa, Jurkat, Mouse testis, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm.
Calculated MW:
51kDa
Observed MW:
60kDa
Involved in several processes, including late endosome to vacuole transport; midbody abscission; and mitotic nuclear division. Located in cytosol; nuclear envelope; and nucleoplasm. Part of ESCRT III complex. Colocalizes with chromatin.
Purification Method
Affinity purification
Gene ID
91782
RRID
AB_2761227
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CHMP7 Rabbit pAb (CAB14360) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
