The BCHE Antibody (CAB1460) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Cholinesterase is crucial for the proper functioning of the nervous system, as it breaks down acetylcholine, a neurotransmitter involved in muscle contractions and cognitive functions. Dysregulation of cholinesterase activity has been linked to various neurological disorders, making it an important target for research in neuroscience and pharmacology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BCHE Antibody
SKU:
CAB1460
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
E1, CHE1, CHE2, BCHED, Butyrylcholinesterase
Positive Sample:
293T, SW620
Cellular Localization:
Secreted.
Calculated MW:
68kDa
Observed MW:
68kDa
This gene encodes a cholinesterase enzyme and member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine.
Purification Method
Affinity purification
Gene ID
590
RRID
AB_2761475
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Butyrylcholinesterase Rabbit pAb (CAB1460) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
