The CIAO3 Polyclonal Antibody (PAC040582) is a powerful tool for researchers studying the CIAO3 protein, a key player in cellular function and immune response modulation. This antibody, produced in rabbits, has high specificity for human samples and has been rigorously validated for use in Western blot applications. By binding to the CIAO3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in immunology and cancer research.
CIAO3, also known as a critical regulator of immune responses, is a crucial component in maintaining immune homeostasis by modulating inflammatory processes and inhibiting aberrant immune reactions. Its role in immune system modulation positions it as a promising target for research into autoimmune disorders, cancer, and chronic inflammatory conditions. Understanding the function of CIAO3 is key to developing novel therapies that manipulate immune responses in these disease contexts.
Western Blot. Positive WB detected in: A549 whole cell lysate. All lanes: CIAO3 antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 27, 43 kDa. Observed band size: 54, 27 kDa.
Immunofluorescent analysis of U251 cells using PACO40582 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human colon cancer using PACO40582 at dilution of 1:100.
Background:
Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.
Synonyms:
Cytosolic iron-sulfur assembly component 3, Cytosolic Fe-S cluster assembly factor NARFL (Iron-only hydrogenase-like protein 1) (IOP1) (Nuclear prelamin A recognition factor-like protein) (Protein related to Narf), CIAO3, NARFL, PRN
UniProt Protein Function:
NARFL: Component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. Belongs to the NARF family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Unknown function
Chromosomal Location of Human Ortholog: 16p13.3
Biological Process: iron-sulfur cluster assembly; oxygen homeostasis; regulation of transcription, DNA-dependent; response to hypoxia
