The Creatine kinase B type (CKB) Antibody (CAB12631) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity and sensitivity towards CKB in human samples, making it ideal for Western blot applications.CKB is involved in the conversion of creatine and ATP to phosphocreatine and ADP, playing a key role in energy transfer in tissues with high and fluctuating energy demands, such as skeletal muscle and brain.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Creatine kinase B type (CKB) Antibody
SKU:
CAB12631
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BCK, B-CK, CKBB, CPK-B, HEL-211, HEL-S-29, Creatine kinase B type (CKB)
Positive Sample:
A-549, HT-29, HeLa, Mouse brain, Rat spinal cord
Cellular Localization:
Cytoplasm.
Calculated MW:
43kDa
Observed MW:
43kDa
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
Purification Method
Affinity purification
Gene ID
1152
RRID
AB_2759476
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Creatine kinase B type (CKB) Rabbit pAb (CAB12631) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
