The Claudin 1 Monoclonal Antibody (CAB21971) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody is specifically designed to target Claudin-1 in human samples, making it an ideal choice for experiments involving cell-cell adhesion, barrier function, and epithelial permeability.Raised in mice, this antibody has been rigorously tested and validated for use in various applications, including immunofluorescence, immunohistochemistry, and flow cytometry. Its high specificity and sensitivity enable precise detection and localization of Claudin-1, allowing researchers to investigate its role in epithelial biology, cancer metastasis, and inflammatory responses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Claudin 1 Monoclonal Antibody
SKU:
CAB21971
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC54475
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
Purification Method
Affinity purification
Gene ID
9076
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] Claudin 1 Rabbit mAb (CAB21971) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using [KO Validated] Claudin 1 Rabbit mAb (CAB21971) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Meriem Cherif et al.
Efficacy of platelet-rich plasma in the treatment of vulvar lichen sclerosus resistant to Clobetasol propionate 0.05%
