The Claudin-16 Polyclonal Antibody (CAB9862) is a valuable tool for researchers studying Claudin-16, a protein involved in maintaining the tight junctions between cells in the kidney. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the Claudin-16 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for studies in nephrology and renal physiology.
Claudin-16 is a crucial component of the tight junctions in the kidney, playing a key role in regulating the movement of ions and molecules across the renal epithelium. Dysregulation of Claudin-16 has been linked to various kidney disorders, including nephrolithiasis and renal hypomagnesemia. Understanding the function and expression of Claudin-16 is essential for furthering our knowledge of renal physiology and developing targeted therapies for kidney diseases.
Product Name:
CLDN16 Rabbit Polyclonal Antibody
SKU:
CAB9862
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CLDN16 (NP_006571.1).
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28.
Purification Method:
Affinity purification
Gene ID:
10686
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CLDN16 antibody (CAB9862) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
