The Claudin-18 Polyclonal Antibody (CAB8392) is a valuable tool for researchers studying Claudin-18, a key protein involved in tight junction formation and barrier function in epithelial cells. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for a variety of research applications including Western blotting.Claudin-18 is a critical component of tight junctions in epithelial tissues, playing a crucial role in maintaining cell polarity and regulating paracellular permeability. Dysregulation of Claudin-18 has been implicated in various diseases, including cancer and gastrointestinal disorders.
By using the Claudin-18 Polyclonal Antibody, researchers can investigate the expression levels and localization of Claudin-18 in different cell types and tissues, providing insights into its role in disease pathology.Overall, the Claudin-18 Polyclonal Antibody is a valuable tool for researchers interested in studying the function and regulation of Claudin-18 in health and disease. Its high specificity and versatility make it an essential reagent for studies aimed at understanding the role of tight junction proteins in epithelial barrier function.
Product Name:
CLDN18 Rabbit Polyclonal Antibody
SKU:
CAB8392
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 200-261 of human CLDN18 (NP_057453.1).
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
51208
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CLDN18 Rabbit pAb (CAB8392) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
