The CLCNKA Antibody (CAB3792) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in various research applications including Western blot and immunohistochemistry.The CLCNKA gene plays a crucial role in maintaining electrolyte balance and renal function, making it a key target for studying kidney disorders and hypertension. By detecting the expression of CLCNKA protein, researchers can gain insights into the function and regulation of chloride channels in the kidney, opening up avenues for potential therapeutic interventions in salt-sensitive hypertension and other related conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CLCNKA Antibody
SKU:
CAB3792
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CLCK1, ClC-K1, hClC-Ka, CLCNKA
Positive Sample:
LO2, HT-29, A-549
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
75kDa
Observed MW:
75kDa
This gene is a member of the CLC family of voltage-gated chloride channels. The encoded protein is predicted to have 12 transmembrane domains, and requires a beta subunit called barttin to form a functional channel. It is thought to function in salt reabsorption in the kidney and potassium recycling in the inner ear. The gene is highly similar to CLCNKB, which is located 10 kb downstream from this gene. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1187
RRID
AB_2765306
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CLCNKA Rabbit pAb (CAB3792) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
