The CNOT4 Polyclonal Antibody (PAC00455) is a vital tool for researchers studying the CNOT4 protein, which plays a crucial role in regulating gene expression and protein degradation processes within the cell. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the CNOT4 protein, it allows for accurate detection and analysis in various cell types, making it an essential component for studies in molecular biology and cellular signaling.
CNOT4 is a key factor in the control of gene expression, acting as a component of the CCR4-NOT complex that regulates mRNA turnover and protein synthesis. Dysfunction of CNOT4 has been linked to various diseases, including cancer and neurological disorders, highlighting its importance in biological processes. Research into the functions of CNOT4 is essential for gaining insights into these diseases and developing targeted therapeutic approaches to modulate its activity.
Antibody Name:
CNOT4 Antibody
Antibody SKU:
PACO04455
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthesized peptide derived from the Internal region of human CNOT4.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
CNOT4: Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex. 8 isoforms of the human protein are produced by alternative splicing.Protein type: Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-; Ligase; Ubiquitin ligaseChromosomal Location of Human Ortholog: 7q33Cellular Component: CCR4-NOT complex; cytosolMolecular Function: protein binding; ubiquitin-protein ligase activityBiological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; poly(A) tail shortening; protein autoubiquitination
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]
