The CNOT8 Antibody (CAB8058) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.The CNOT8 protein is a component of the CCR4-NOT complex, which is involved in mRNA deadenylation and degradation. By targeting CNOT8, researchers can investigate its role in post-transcriptional gene regulation and its impact on cellular processes such as cell proliferation, differentiation, and apoptosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CNOT8 Antibody
SKU:
CAB8058
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAF1, POP2, CALIF, Caf1b, hCAF1, CNOT8
Positive Sample:
293T, Mouse liver, Mouse brain
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
34kDa
Observed MW:
34kDa
Enables poly(A)-specific ribonuclease activity. Involved in exonucleolytic catabolism of deadenylated mRNA and positive regulation of cell population proliferation. Located in nucleus. Part of CCR4-NOT complex.
Purification Method
Affinity purification
Gene ID
9337
RRID
AB_2768973
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CNOT8 Rabbit pAb (CAB8058) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
