The CNPase Antibody (CAB1018) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity. Involved in substantia nigra development. Located in several cellular components, including extracellular space; microtubule; and plasma membrane. Implicated in hypomyelinating leukodystrophy 20; multiple sclerosis; and schizophrenia. Biomarker of alcoholic liver cirrhosis; multiple sclerosis; and restless legs syndrome. RRID AB_2757702 Gene ID 1267 Swiss Prot Synonym CNP1; HLD20; CNPase
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CNPase Antibody
SKU:
CAB1018
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CNP1, HLD20, CNPase
Positive Sample:
HepG2, 293T, Rat brain, Mouse brain, Hep G2, A-375
Cellular Localization:
Lipid-Anchor, Melanosome, Membrane.
Calculated MW:
48kDa
Observed MW:
48kDa/
Predicted to enable 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity. Involved in substantia nigra development. Located in several cellular components, including extracellular space; microtubule; and plasma membrane. Implicated in hypomyelinating leukodystrophy 20; multiple sclerosis; and schizophrenia. Biomarker of alcoholic liver cirrhosis; multiple sclerosis; and restless legs syndrome. RRID AB_2757702 Gene ID 1267 Swiss Prot Synonym CNP1; HLD20; CNPase
Purification Method:
Affinity purification
Gene ID:
1267
RRID:
AB_2757702
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CNPase Rabbit pAb (CAB1018) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of paraffin-embedded rat brain using CNPasease Rabbit pAb (CAB1018) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
