The COASY Antibody (CAB12179) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blotting applications.COASY is essential for cellular energy metabolism and is involved in various physiological processes, making it a key target for research in metabolic disorders, neurodegenerative diseases, and cancer. By binding specifically to the COASY protein, this polyclonal antibody enables precise detection and analysis in a range of cell types, making it an ideal choice for investigations in biochemistry and molecular biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
COASY Antibody
SKU:
CAB12179
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NBP, DPCK, PPAT, UKR1, NBIA6, PCH12, pOV-2, CoA synthase (COASY）
Positive Sample:
LO2, 293T, A-549, SKOV3, Mouse heart, Mouse liver, Rat kidney
Cellular Localization:
Cytoplasm, Mitochondrion Matrix.
Calculated MW:
62kDa
Observed MW:
62kDa
Coenzyme A (CoA) functions as a carrier of acetyl and acyl groups in cells and thus plays an important role in numerous synthetic and degradative metabolic pathways in all organisms. In eukaryotes, CoA and its derivatives are also involved in membrane trafficking and signal transduction. This gene encodes the bifunctional protein coenzyme A synthase (CoAsy) which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5). The phosphopantetheine adenylyltransferase domain of this bifunctional protein catalyzes the conversion of 4'-phosphopantetheine into dephospho-coenzyme A (dpCoA) while its dephospho-CoA kinase domain completes the final step by phosphorylating dpCoA to form CoA. Mutations in this gene are associated with neurodegeneration with brain iron accumulation (NBIA). Alternative splicing results in multiple isoforms.
Purification Method
Affinity purification
Gene ID
80347
RRID
AB_2759066
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CoA synthase (COASY） Rabbit pAb (CAB12179) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
