The Coilin Monoclonal Antibody (CAB3483) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is ideal for studies in cell biology, specifically focusing on nuclear organization and RNA processing.Coilin is a key component of Cajal bodies, specialized sub-nuclear structures involved in various cellular processes such as RNA transcription, splicing, and telomere maintenance. Dysfunction of coilin has been implicated in diseases like spinal muscular atrophy and cancer, making it an important target for research in these areas.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Coilin Monoclonal Antibody
SKU:
CAB3483
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC0785
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CLN80, p80-coilin, Coilin
Positive Sample:
HeLa, Mouse lung
Cellular Localization:
Cajal Body, Nucleus.
Calculated MW:
63kDa
Observed MW:
80kDa
The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.
Purification Method
Affinity purification
Gene ID
8161
RRID
AB_2863068
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Coilin Rabbit mAb (CAB3483) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
