The Coilin Antibody (CAB6428) is a high-quality antibody developed for reliable detection and analysis of target proteins. Cajal bodies are important subnuclear structures involved in RNA processing and ribonucleoprotein biogenesis.Raised in rabbits, this antibody is highly specific and reactive to human samples, making it ideal for various research applications. It has been validated for use in Western blotting, immunofluorescence, and immunohistochemistry, allowing for the detection and analysis of coilin in different cell types and tissues.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Coilin Antibody
SKU:
CAB6428
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CLN80, p80-coilin, Coilin
Positive Sample:
MCF7, HepG2, 293T, Rat testis
Cellular Localization:
Cajal Body, Nucleus.
Calculated MW:
63kDa
Observed MW:
80kDa
The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.
Purification Method
Affinity purification
Gene ID
8161
RRID
AB_2767030
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Coilin Rabbit pAb (CAB6428) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
