The COL4A5 Antibody (CAB9809) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically targets the COL4A5 protein and is highly reactive with human samples. Validated for use in Western blot applications, it enables precise detection and analysis of COL4A5 in different cell types.Type IV collagen is essential for the structural integrity of basement membranes and plays a crucial role in cell adhesion, migration, and differentiation. Dysregulation of COL4A5 expression has been associated with various diseases, including Alport syndrome, a genetic disorder affecting the kidneys and eyes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
COL4A5 Antibody
SKU:
CAB9809
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene.
Purification Method
Affinity purification
Gene ID
1287
RRID
AB_2768996
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using COL4A5 Rabbit pAb (CAB9809) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of A-431 cells using COL4A5 Rabbit pAb (CAB9809) at dilution of 1:1000 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
