The COL8A2 Monoclonal Antibody (PAC07443) is a valuable tool for researchers studying collagen type VIII alpha 2 chain (COL8A2), a key component of extracellular matrix organization. This antibody, produced by Assay Genie, is specific to the COL8A2 protein and is suitable for use in various experimental applications, including immunohistochemistry and western blotting.COL8A2 is a crucial structural protein involved in maintaining tissue integrity and function, particularly in the cornea and other connective tissues. Dysregulation of COL8A2 expression has been linked to various diseases, including corneal dystrophies and cardiovascular disorders.
By using the PAC07443 antibody, researchers can accurately detect and analyze COL8A2 levels in different tissue samples, aiding in the understanding of its role in disease development and progression.With its high specificity and sensitivity, the COL8A2 Monoclonal Antibody (PAC07443) is an essential tool for investigating the function and significance of COL8A2 in various biological processes. Its applications extend to studies in ophthalmology, cardiovascular research, and tissue engineering, providing valuable insights into the molecular mechanisms underlying these conditions.
Antibody Name:
COL8A2 Antibody (PACO07443)
Antibody SKU:
PACO07443
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:10000-1:20000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from human COL8A2. at AA range: 611-660
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of 1,hela 2,3T3 cells using primary antibody diluted at 1:500(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour).
Synonyms:
Collagen α-2(VIII) chain (Endothelial collagen)
UniProt Protein Function:
COL8A2: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis. Defects in COL8A2 are the cause of corneal dystrophy Fuchs endothelial type 1 (FECD1). It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. Defects in COL8A2 are the cause of posterior polymorphous corneal dystrophy type 2 (PPCD2). PPCD is a rare bilateral familial disorder of the corneal epithelium, and is inherited in a autosomal dominant pattern. The clinical features usually present earlier than FECD, being from birth onwards. The disorder is characterized by alterations of Descemet membrane presenting as vesicles, opacities or band-like lesions on slit- lamp examination and specular microscopy. Affected patient typically are asymptomatic.Protein type: Secreted, signal peptide; Secreted; Extracellular matrixChromosomal Location of Human Ortholog: 1p34.2Cellular Component: endoplasmic reticulum lumen; extracellular matrix; extracellular regionBiological Process: collagen catabolic process; extracellular matrix organization and biogenesisDisease: Corneal Dystrophy, Fuchs Endothelial, 1; Corneal Dystrophy, Posterior Polymorphous, 2
UniProt Protein Details:
NCBI Summary:
This gene encodes the alpha 2 chain of type VIII collagen. This protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
