Complement factor D Monoclonal Antibody (CAB23006)
The Complement factor D Monoclonal Antibody (CAB23006) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, specifically targets and binds to complement factor D in human samples, allowing for precise detection and analysis in a variety of research applications.Complement factor D is a crucial component of the immune response, playing a significant role in the activation and regulation of the complement system. Dysregulation of this pathway has been implicated in numerous diseases, including inflammatory disorders, autoimmune conditions, and infectious diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Complement factor D Monoclonal Antibody
SKU:
CAB23006
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC59438
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:10000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DF, ADN, PFD, ADIPSIN, Complement factor D
Positive Sample:
THP-1
Cellular Localization:
Secreted.
Calculated MW:
27kDa
Observed MW:
27kDa
This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease.
Purification Method
Affinity purification
Gene ID
1675
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Complement factor D Rabbit mAb (CAB23006) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
