Description
COPA Antibody (CAB16821)
The COPA Antibody (CAB16821) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the COPA protein, this antibody allows for accurate detection and analysis in various cell types, making it well-suited for studies in immunology and cell biology.COPA, a key component of the coatomer protein complex, plays a crucial role in intracellular transport and the maintenance of cell structure.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
| Product Name: | COPA Antibody |
| SKU: | CAB16821 |
| Size: | 20μL, 100μL |
| Reactivity: | Mouse |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | FIYT TSNH IKYA VTTG DHGI IRTL DLPI YVTR VKGN NVYC LDRE CRPR VLTI DPTE FKFK LALI NRKY DEVL HMVR NAKL VGQS IIAY LQKK GYPE VALH F | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | AILJK, HEP-COP, alpha-COP, COPA |
| Positive Sample: | Mouse liver |
| Cellular Localization: | Copi Vesicle Coat, Cytoplasm, Cytosol, Extracellular Exosome, Extracellular Space, Transport Vesicle. |
| Calculated MW: | 138kDa |
| Observed MW: | 110kDa |
In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms.
| Purification Method | Affinity purification |
| Gene ID | 1314 |
| RRID | AB_2769007 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Mouse liver, using COPA Rabbit pAb (CAB16821) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s. |
