The Cox17 Antibody (PAC023780) is a crucial tool for researchers investigating the function of Cox17, a key protein involved in copper homeostasis and mitochondrial function. This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting Cox17 in human samples, making it ideal for use in Western blotting and immunohistochemistry experiments.Cox17 is essential for the delivery of copper ions to cytochrome c oxidase, the terminal enzyme in the mitochondrial electron transport chain.
Dysregulation of Cox17 has been linked to mitochondrial dysfunction and oxidative stress, contributing to various diseases such as neurodegenerative disorders and metabolic syndromes. By targeting Cox17 with this antibody, researchers can explore its role in cellular metabolism, energy production, and oxidative stress response, providing insights into potential therapeutic strategies for mitochondrial-related diseases.
Synthesized peptide derived from N-terminal of human COX17.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using COX17 antibody.
Western blot analysis of extracts from 3T3 cells (Lane 2), using COX17 antiobdy. The lane on the left is treated with synthesized peptide.
Western blot analysis of extracts from HeLa cells, HepG2 cells, COLO cells and 293 cells, using COX17 antibody.
Background:
Copper chaperone for cytochrome c oxidase (COX). Binds two copper ions and deliver them to the Cu(A) site of COX By similarity.
Synonyms:
Cytochrome c oxidase copper chaperone; COX17;
UniProt Protein Function:
COX17: Copper chaperone for cytochrome c oxidase (COX). Binds two copper ions and deliver them to the Cu(A) site of COX. Belongs to the COX17 family.Protein type: Mitochondrial; Energy Metabolism - oxidative phosphorylationChromosomal Location of Human Ortholog: 3q13.33Cellular Component: mitochondrion; cytoplasm; mitochondrial intermembrane spaceMolecular Function: copper ion binding; copper chaperone activityBiological Process: generation of precursor metabolites and energy; heart development; brain development; copper ion transport
UniProt Protein Details:
NCBI Summary:
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be involved in the recruitment of copper to mitochondria for incorporation into the COX apoenzyme. This protein shares 92% amino acid sequence identity with mouse and rat Cox17 proteins. This gene is no longer considered to be a candidate gene for COX deficiency. A pseudogene COX17P has been found on chromosome 13. [provided by RefSeq, Jul 2008]
