The COX6B2 Polyclonal Antibody (PAC014280) is a valuable tool for researchers studying the role of the COX6B2 protein in cellular respiration and mitochondrial function. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the COX6B2 protein, this antibody allows for accurate detection and analysis of COX6B2 expression in various cell types, making it ideal for investigations in the fields of bioenergetics and mitochondrial biology.COX6B2 is a subunit of cytochrome c oxidase, the terminal enzyme complex in the mitochondrial electron transport chain responsible for ATP production through oxidative phosphorylation.
As such, COX6B2 plays a critical role in energy metabolism and cellular respiration. Dysregulation of COX6B2 expression or function has been implicated in a variety of diseases, including metabolic disorders and neurodegenerative conditions. Research into the molecular mechanisms underlying COX6B2 activity is essential for developing targeted therapies aimed at correcting mitochondrial dysfunction and restoring cellular energy balance.
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO14280(COX6B2 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200).
Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: A549 cells, Primary antibody: PACO14280(COX6B2 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO14280(COX6B2 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200).
Background:
The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Localized to the intermembrane space, COX6b2 (Cytochrome c oxidase subunit 6B2), also known as Cytochrome c oxidase subunit VIb isoform 2 and Cancer/testis antigen 59, is a 88 amino acid, mitochondrial protein that is responsible for joining the two COX monomers to form the COX dimer. COX6b2 is specifically expressed in testis and is found to be upregulated in certain cancer cell lines.
Synonyms:
cytochrome c oxidase subunit VIb polypeptide 2 (testis)
UniProt Protein Function:
COX6B2: Connects the two COX monomers into the physiological dimeric form. Belongs to the cytochrome c oxidase subunit 6B family.
UniProt Protein Details:
Protein type:Oxidoreductase; Cancer Testis Antigen (CTA); Mitochondrial
Chromosomal Location of Human Ortholog: 19q13.42
Cellular Component: mitochondrial crista; mitochondrial intermembrane space
