The CPSF1 Antibody (CAB17144) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot and immunofluorescence applications.CPSF1 is a critical component of the Cleavage and Polyadenylation Specificity Factor (CPSF) complex, which plays a crucial role in the cleavage and polyadenylation of mRNA molecules. Dysregulation of CPSF1 has been linked to various diseases, including cancer and neurological disorders, making it a valuable target for further research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CPSF1 Antibody
SKU:
CAB17144
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MYP27, CPSF160, P/cl.18, HSU37012, CPSF1
Positive Sample:
293T, NIH/3T3, Mouse liver
Cellular Localization:
Nucleoplasm, Nucleus.
Calculated MW:
161kDa
Observed MW:
170kDa/185kDa
Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).
Purification Method
Affinity purification
Gene ID
29894
RRID
AB_2769032
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CPSF1 Rabbit pAb (CAB17144) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
