The CRAT Antibody (CAB6365) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.CRAT plays a crucial role in the transportation of long-chain fatty acids into the mitochondria for β-oxidation, a key process in energy production. Dysregulation of CRAT has been implicated in metabolic disorders, including obesity and fatty liver disease, making it an important target for research in the fields of metabolism and energy balance.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CRAT Antibody
SKU:
CAB6365
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes carnitine O-acetyltransferase, a member of the carnitine acyltransferase family and a key metabolic pathway enzyme which plays an important role in energy homeostasis and fat metabolism. This enzyme catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acyl-CoA/CoA. It is found in both the mitochondria and the peroxisome. Alternative splicing results in transcript variants encoding different isoforms that may localize to different subcellular compartments.
Purification Method
Affinity purification
Gene ID
1384
RRID
AB_2766967
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CRAT Rabbit pAb (CAB6365) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of RD cells using CRAT Rabbit pAb (CAB6365) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
