The CROT Antibody (CAB4790) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, boasts high reactivity with human samples and is validated for use in Western blot applications. By binding to the CROT protein, this antibody allows for the accurate detection and analysis of CROT in various cell types, making it an ideal choice for studies in metabolism, energy regulation, and mitochondrial function.CROT, also known as carnitine O-octanoyltransferase, plays a crucial role in fatty acid β-oxidation, the process by which fatty acids are broken down to generate energy.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CROT Antibody
SKU:
CAB4790
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 25μg-100μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
COT, CROT
Positive Sample:
Rat liver
Cellular Localization:
Peroxisome.
Calculated MW:
70kDa
Observed MW:
70kDa
This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
54677
RRID
AB_2765868
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from rat liver, using CROT Rabbit pAb (CAB4790) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
