The CRYAA Antibody (CAB5725) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is specifically reactive with human samples and has been validated for use in Western blot applications. By binding to the CRYYA protein, researchers can study its expression and function in various cell types, making it a valuable tool for studies in ophthalmology and developmental biology.CRYYA, also referred to as Alpha-Crystallin A Chain, plays a crucial role in maintaining the transparency and refractive index of the lens in the eye.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat, Zebrafish samples.
Product Name:
CRYAA Antibody
SKU:
CAB5725
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CRYA1, HSPB4, CTRCT9, CRYAA
Positive Sample:
HepG2
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
20kDa
Observed MW:
19kDa
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).
Purification Method
Affinity purification
Gene ID
1409
RRID
AB_2766482
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HepG2 cells, using CRYAA Rabbit pAb (CAB5725) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded Rat Eye using CRYAA Rabbit pAb (CAB5725) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
