The Cystathionase/CTH Antibody (CAB6121) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the CTH protein, this antibody enables the detection and analysis of CTH in various cell types, making it ideal for studies in biochemistry and molecular biology.CTH, also known as cystathionine gamma-lyase, plays a crucial role in the transsulfuration pathway and the production of hydrogen sulfide, an important signaling molecule in the body.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cystathionase/CTH Antibody
SKU:
CAB6121
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CGL, CSE, Cystathionase/CTH
Positive Sample:
K-562, Mouse liver, Mouse kidney, Rat liver, Rat kidney
Cellular Localization:
Cytoplasm.
Calculated MW:
45kDa
Observed MW:
45kDa
This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
1491
RRID
AB_2766754
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Cystathionase/CTH Rabbit pAb (CAB6121) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of HepG2 cells using Cystathionase/CTH Rabbit pAb (CAB6121) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
