The CTSK Antibody (CAB1782) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbits as hosts, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the Cathepsin K protein, researchers can accurately detect and analyze its expression in various cell types, making it particularly valuable for studies in osteoporosis, arthritis, and other bone-related diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CTSK Antibody
SKU:
CAB1782
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CTSO, PKND, PYCD, CTS02, CTSO1, CTSO2, CTSK
Positive Sample:
SK-MEL-28, U-87MG, MG-63
Cellular Localization:
Lysosome.
Calculated MW:
37kDa
Observed MW:
26kDa/37kDa
The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature.
Purification Method
Affinity purification
Gene ID
1513
RRID
AB_2763824
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CTSK Rabbit pAb (CAB1782) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
