The Cortactin Antibody (CAB5795) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the CTTN protein, this antibody enables researchers to detect and analyze CTTN expression in various cell types, making it an essential tool for studies in cancer biology and metastasis research.CTTN, also known as cortactin, plays a crucial role in regulating the actin cytoskeleton and promoting cell motility.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cortactin Antibody
SKU:
CAB5795
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.
Purification Method
Affinity purification
Gene ID
2017
RRID
AB_2766547
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cortactin Rabbit pAb (CAB5795) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.5s.
Western blot analysis of lysates from Mouse kidney, using Cortactin Rabbit pAb (CAB5795) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of PC-12 using Cortactin Rabbit pAb (CAB5795) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa using Cortactin Rabbit pAb (CAB5795) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
