The CUL4B Antibody (CAB12696) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity in detecting CUL4B in human samples, making it a reliable tool for Western blot applications.CUL4B is a member of the cullin family of proteins and functions as a scaffold protein in the CUL4-RING E3 ubiquitin ligase complex, playing a key role in targeting specific substrate proteins for degradation. Dysregulation of CUL4B has been linked to various diseases, including cancer and neurodevelopmental disorders, highlighting the importance of studying its function and regulatory mechanisms.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CUL4B Antibody
SKU:
CAB12696
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SFM2, MRXSC, CUL-4B, MRXHF2, MRXS15, CUL4B
Positive Sample:
SH-SY5Y, HeLa, 293T
Cellular Localization:
Nucleus.
Calculated MW:
104kDa
Observed MW:
105kDa
This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
8450
RRID
AB_2759539
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CUL4B Rabbit pAb (CAB12696) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
