The CWC22 Antibody (CAB13116) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables RNA binding activity. Involved in mRNA splicing, via spliceosome. Located in cytosol and nuclear speck. Part of U2-type catalytic step 1 spliceosome; U2-type catalytic step 2 spliceosome; and U2-type precatalytic spliceosome. RRID AB_2759965 Gene ID 57703 Swiss Prot Synonym NCM; fSAPb; EIF4GL; CWC22
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
CWC22 Antibody
SKU:
CAB13116
Size:
100μL, 20μL
Reactivity:
Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NCM, fSAPb, EIF4GL, CWC22
Positive Sample:
Mouse liver, Mouse testis
Cellular Localization:
Nucleus, Nucleus Speckle.
Calculated MW:
105kDa
Observed MW:
110kDa
Enables RNA binding activity. Involved in mRNA splicing, via spliceosome. Located in cytosol and nuclear speck. Part of U2-type catalytic step 1 spliceosome; U2-type catalytic step 2 spliceosome; and U2-type precatalytic spliceosome. RRID AB_2759965 Gene ID 57703 Swiss Prot Synonym NCM; fSAPb; EIF4GL; CWC22
Purification Method:
Affinity purification
Gene ID:
57703
RRID:
AB_2759965
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CWC22 Rabbit pAb (CAB13116) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
