The CXCR5 Monoclonal Antibody (CAB8950) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using rabbit monoclonal technology, exhibits high specificity and sensitivity for CXCR5 in human samples, making it a reliable choice for various immunological studies.CXCR5, also known as CD185, is known to be expressed on B cells, T follicular helper cells, and subsets of T cells, where it regulates their migration towards B cell follicles and germinal centers. Its role in orchestrating immune cell interactions within secondary lymphoid organs makes it a key target for research into autoimmune diseases, infectious diseases, and cancer.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CXCR5 Monoclonal Antibody
SKU:
CAB8950
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1363
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BLR1, CD185, MDR15, CXCR5
Positive Sample:
Jurkat, K-562, Mouse lung, Mouse spleen, Mouse thymus, Rat thymus
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Purification Method
Affinity purification
Gene ID
643
RRID
AB_2863634
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CXCR5 Rabbit mAb (CAB8950) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Confocal imaging of human tonsil using CXCR5 Rabbit mAb (CAB8950,dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
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