The Cyclin H Polyclonal Antibody (CAB21703) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting Cyclin H in human samples, making it ideal for use in Western blot applications. By targeting the Cyclin H protein, this antibody allows for precise and reliable analysis of cell cycle dynamics in various cell types.Cyclin H is essential for the proper functioning of cyclin-dependent kinases, crucial enzymes involved in cell division. Dysregulation of Cyclin H has been implicated in various diseases, including cancer, making it a promising therapeutic target.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Cyclin H Polyclonal Antibody
SKU:
CAB21703
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAK, p34, p37, CycH, Cyclin H
Positive Sample:
Jurkat, K-562, SW620, HT-29, MCF7
Cellular Localization:
Nucleus.
Calculated MW:
38kDa
Observed MW:
Refertofigures
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
902
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cyclin H Rabbit pAb (CAB21703). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020).
