The CYP8B1 Polyclonal Antibody (PACO00673) is a valuable tool for researchers studying the CYP8B1 enzyme, which plays a key role in bile acid biosynthesis. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the CYP8B1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it essential for investigations in the fields of metabolism and liver disease.CYP8B1 is a crucial enzyme involved in the conversion of cholesterol to bile acids, which are essential for lipid digestion and absorption.
Dysregulation of CYP8B1 activity has been linked to disorders such as cholestasis and gallstone formation, highlighting the importance of studying this enzyme in the context of liver health. Furthermore, CYP8B1 inhibitors are being explored as potential therapeutic targets for metabolic disorders and liver diseases, emphasizing the relevance of research using the CYP8B1 Polyclonal Antibody.
Antibody Name:
CYP8B1 Antibody
Antibody SKU:
PACO00673
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human, Mouse
Immunogen:
synthesized peptide derived from the Internal region of human CYP8B1.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
CYP8B1: Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids. Belongs to the cytochrome P450 family.Protein type: Lipid Metabolism - primary bile acid biosynthesis; Membrane protein, integral; EC 1.14.13.95; OxidoreductaseChromosomal Location of Human Ortholog: 3p22.1Cellular Component: endoplasmic reticulum membraneMolecular Function: 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity; oxygen binding; sterol 12-alpha-hydroxylase activityBiological Process: bile acid biosynthetic process; sterol metabolic process
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008]
