The Cytokeratin 19 (KRT19) Monoclonal Antibody (CAB19040) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. RRID AB_2862533 Gene ID 3880 Swiss Prot Synonym K19; KRT19; K1CS; Cytokeratin 19 (CK19)
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human samples.
Product Name:
Cytokeratin 19 (KRT19) Monoclonal Antibody
SKU:
CAB19040
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC2811
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISAIF-P
Recommended Dilution:
WB
1:500 - 1:3000
IF
/
ICC
1:100 - 1:400
IF-P
1:100 - 1:400
IHC-P
1:2000 - 1:8000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
K19, KRT19, K1CS, Cytokeratin 19 (CK19)
Positive Sample:
BxPC-3, MCF7
Cellular Localization:
Cytoplasm.
Calculated MW:
44 kDa
Observed MW:
44 kDa
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. RRID AB_2862533 Gene ID 3880 Swiss Prot Synonym K19; KRT19; K1CS; Cytokeratin 19 (CK19)
Purification Method:
Affinity purification
Gene ID:
3880
RRID:
AB_2862533
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cytokeratin 19 (CK19) Rabbit mAb (CAB19040) at 1:500 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): HeLa Exposure time: 0.5s.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Cytokeratin 19 (CK19) Rabbit mAb (CAB19040) at a dilution of 1:2500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Cytokeratin 19 (CK19) Rabbit mAb (CAB19040) at a dilution of 1:2500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of MCF7 cells using Cytokeratin 19 (CK19) Rabbit mAb (CAB19040, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
