The Cytokeratin 7 (KRT7) Monoclonal Antibody (CAB4357) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and is validated for use in various research applications, including immunohistochemistry and Western blotting.Cytokeratin 7 is a member of the cytokeratin family of proteins, which are essential components of the cytoskeleton and play a key role in maintaining cell integrity. It is commonly found in epithelial cells, making it a useful marker for identifying various types of cancers, such as breast, lung, and ovarian cancer.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cytokeratin 7 (KRT7) Monoclonal Antibody
SKU:
CAB4357
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0978
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
K7, KRT7, SCL, K2C7, Cytokeratin 7 (CK7)
Positive Sample:
HeLa, Mouse lung
Cellular Localization:
Cytoplasm.
Calculated MW:
51kDa
Observed MW:
55kDa
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
Purification Method
Affinity purification
Gene ID
3855
RRID
AB_2863248
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cytokeratin 7 (KRT7) Rabbit mAb (CAB4357)at 1:20000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): MCF-7 Exposure time: 1s.
Immunofluorescence analysis of paraffin-embedded Human lung tissue using Cytokeratin 7 (KRT7) Rabbit mAb (CAB4357) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of paraffin-embedded Rat lung tissue using Cytokeratin 7 (KRT7) Rabbit mAb (CAB4357) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
