The DACH1 Antibody (CAB6895) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to Dachshund Homolog 1, it enables researchers to detect and analyze this protein in various cell types, making it an excellent choice for studies in developmental biology and cancer research.Dachshund Homolog 1, also known as DACH1, is a transcription factor that plays a crucial role in controlling gene expression patterns that are essential for proper cell differentiation and tissue development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DACH1 Antibody
SKU:
CAB6895
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DACH, DACH1
Positive Sample:
293F
Cellular Localization:
Nucleus.
Calculated MW:
79kDa
Observed MW:
75kDa
This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1602
RRID
AB_2767454
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293F cells, using DACH1 Rabbit pAb (CAB6895) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
