The DACH2 polyclonal antibody (PACO16136) is designed for research involving DACH2, a transcription factor that plays a critical role in development and disease. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in various applications, including immunohistochemistry and Western blotting. By binding to the DACH2 protein, researchers can analyze its expression and function in different cell types, making it an essential tool for studying developmental biology and cancer research.DACH2, a member of the DACH family of transcription factors, is known to regulate gene expression during embryonic development and has been implicated in various diseases, including cancer.
Its involvement in cell differentiation and tissue growth makes it a key player in understanding developmental processes and identifying potential therapeutic targets for treating developmental disorders and cancer. By studying the function of DACH2, researchers can unravel its role in normal development and disease pathogenesis, paving the way for innovative treatments and interventions in the future.
Antibody Name:
DACH2 Antibody (PACO16136)
Antibody SKU:
PACO16136
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human DACH2
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO16136(DACH2 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO16136(DACH2 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200).
Background:
This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for this gene.
Synonyms:
dachshund homolog 2 (Drosophila)
UniProt Protein Function:
DACH2: Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain. Belongs to the DACH/dachshund family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: DNA-bindingChromosomal Location of Human Ortholog: Xq21.3Cellular Component: nucleus; transcription factor complexBiological Process: multicellular organismal development; regulation of transcription, DNA-dependent
UniProt Protein Details:
NCBI Summary:
This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
