The DAO Antibody (CAB5309) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to DAO, researchers can detect and analyze the enzyme in various cell types, making it an ideal choice for studies in immunology, allergy research, and gastrointestinal disorders.
This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DAO Antibody
SKU:
CAB5309
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DAAO, OXDA, DAMOX, DAO
Positive Sample:
Rat kidney
Cellular Localization:
Peroxisome.
Calculated MW:
39kDa
Observed MW:
39kDa
This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia.
Purification Method
Affinity purification
Gene ID
1610
RRID
AB_2766121
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat kidney, using DAO Rabbit pAb (CAB5309) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Confocal immunofluorescence analysis of L929 cells using DAO Rabbit pAb (CAB5309) at dilution of 1:100. Blue: DAPI for nuclear staining.
