The DCTD Antibody (CAB5889) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in various applications, including Western blotting. By binding to the DCTD protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in molecular biology and cancer research.DCTD, also known as deoxycytidylate deaminase, plays a crucial role in maintaining genomic stability and integrity by ensuring accurate nucleotide incorporation during DNA replication.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DCTD Antibody
SKU:
CAB5889
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DCTD
Positive Sample:
SW620, Mouse heart, Rat heart
Cellular Localization:
Cytosol.
Calculated MW:
20kDa
Observed MW:
23kDa
The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1635
RRID
AB_2766637
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DCTD Rabbit pAb (CAB5889) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using DCTD Rabbit pAb (CAB5889) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
