The DDX6 Antibody (CAB16270) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for Western blot applications.DDX6, also referred to as RCK/p54, is a member of the DEAD-box RNA helicase family and plays a crucial role in post-transcriptional gene regulation. Its functions in mRNA decay and processing make it a key player in cellular processes such as RNA splicing, transport, and translation. By targeting DDX6 with this polyclonal antibody, researchers can accurately detect and analyze its expression levels in various cell types, providing valuable insights into its role in gene expression control.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DDX6 Antibody
SKU:
CAB16270
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P54, RCK, HLR2, IDDILF, Rck/p54, DDX6
Positive Sample:
HepG2, HeLa, NIH/3T3, Rat spleen
Cellular Localization:
Cytoplasm, P-Body.
Calculated MW:
54kDa
Observed MW:
54kDa
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Purification Method
Affinity purification
Gene ID
1656
RRID
AB_2769140
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DDX6 Rabbit pAb (CAB16270) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using DDX6 Rabbit pAb (CAB16270) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DDX6 Rabbit pAb (CAB16270) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of L929 cells using DDX6 Rabbit pAb (CAB16270) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
