The DEPDC6 Antibody (CAB9447) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly sensitive and has been validated for use in various applications, including Western blotting and immunohistochemistry.DEPDC6, also known as DEP domain containing 6, is a key player in cellular processes such as cell proliferation, differentiation, and apoptosis. Its role in regulating critical signaling pathways makes it a promising target for studies in cancer research and developmental biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
DEPDC6 Antibody
SKU:
CAB9447
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DEP.6, DEPDC6
Positive Sample:
HeLa, HepG2, MCF7
Calculated MW:
46kDa
Observed MW:
48kDa
Involved in several processes, including negative regulation of TOR signaling; negative regulation of cell size; and negative regulation of protein kinase activity.
Purification Method
Affinity purification
Gene ID
64798
RRID
AB_2769149
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DEPDC6 Rabbit pAb (CAB9447) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
