The DET1 Antibody (CAB9974) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with plant samples, making it an essential tool for researchers studying photomorphogenesis and plant development.DET1 is a key regulator of light-dependent development processes in plants, including seed germination, seedling growth, and flowering. By targeting DET1 with this antibody in experiments such as Western blot analysis, researchers can gain valuable insights into the molecular mechanisms underlying light perception and response in plants.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DET1 Antibody
SKU:
CAB9974
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DET1
Positive Sample:
BT-474, Mouse thymus
Cellular Localization:
Nucleus.
Calculated MW:
64kDa
Observed MW:
55kDa
Enables ubiquitin ligase-substrate adaptor activity and ubiquitin protein ligase binding activity. Involved in positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; and protein-containing complex assembly. Part of Cul4A-RING E3 ubiquitin ligase complex.
Purification Method
Affinity purification
Gene ID
55070
RRID
AB_2769152
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DET1 Rabbit pAb (CAB9974) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
