The DGKZ Antibody (CAB16457) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. RRID AB_2769157 Gene ID 8525 Swiss Prot Synonym DAGK5; DAGK6; DGK-ZETA; hDGKzeta; DGKZ
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
DGKZ Antibody
SKU:
CAB16457
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DAGK5, DAGK6, DGK-ZETA, hDGKzeta, DGKZ
Positive Sample:
LO2, HeLa, 293T, Mouse brain
Cellular Localization:
Cell Membrane, Cytoplasm, Nucleus.
Calculated MW:
104kDa
Observed MW:
120kDa
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. RRID AB_2769157 Gene ID 8525 Swiss Prot Synonym DAGK5; DAGK6; DGK-ZETA; hDGKzeta; DGKZ
Purification Method:
Affinity purification
Gene ID:
8525
RRID:
AB_2769157
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DGKZ Rabbit pAb (CAB16457) at 1:300 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
