Description
Anti-DGUOK Antibody (CAB19926)[KO Validated]
The DGUOK Polyclonal Antibody (CAB19926) is a valuable tool for researchers studying the DGUOK protein, an essential enzyme involved in mitochondrial DNA replication. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it an ideal choice for Western blot applications. By binding to the DGUOK protein, this antibody enables precise detection and analysis in various cell types, facilitating research in mitochondrial biology, DNA replication, and related diseases.DGUOK plays a critical role in maintaining mitochondrial genome integrity and function, making it a key target for understanding mitochondrial diseases, such as hepatocerebral mitochondrial DNA depletion syndrome.
Research on DGUOK is essential for unraveling the mechanisms underlying these diseases and developing potential therapeutic strategies. The validation of this antibody for Western blot applications ensures reliable and accurate results for researchers studying the role of DGUOK in health and disease.
| Antibody Name: | Anti-DGUOK Antibody [KO Validated] |
| Antibody SKU: | CAB19926 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human DGUOK. |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T |
| Immunogen: | Recombinant protein of human DGUOK. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 1716 |
| Uniprot: | Q16854 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 9kDa/17kDa/21kDa/27kDa/29kDa/32kDa |
| Observed MW: | 45KDa |
![Anti-DGUOK Antibody [KO Validated] (CAB19926)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/h/694/A19926_1__25564.jpg) | Western blot analysis of extracts from normal (control) and DGUOK knockout (KO) 293T cells, using DGUOK antibody (CAB19926) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
| Synonyms: | DGUOK, MTDPS3, NCPH, PEOB4, dGK |
| Background: | In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene. |
| UniProt Protein Function: | DGUOK: Required for the phosphorylation of several deoxyribonucleosides and certain nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Defects in DGUOK are the cause of mitochondrial DNA depletion syndrome type 3 (MTDPS3). A disorder characterized by onset in infancy of progressive liver failure, hypoglycemia, increased lactate in body fluids, and neurologic abnormalities including hypotonia, encephalopathy, peripheral neuropathy. Affected tissues show both decreased activity of the mtDNA-encoded respiratory chain complexes and mtDNA depletion. Belongs to the DCK/DGK family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - purine; EC 2.7.1.113; Mitochondrial; Kinase, other Chromosomal Location of Human Ortholog: 2p13 Cellular Component: mitochondrion; mitochondrial matrix; nucleus; cytosol Molecular Function:deoxyguanosine kinase activity; nucleoside kinase activity; ATP binding Biological Process: purine deoxyribonucleoside metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; dGTP metabolic process; guanosine metabolic process; protein amino acid phosphorylation; purine salvage; purine base metabolic process; deoxyribonucleoside monophosphate biosynthetic process Disease: Mitochondrial Dna Depletion Syndrome 3 (hepatocerebral Type) |
| NCBI Summary: | In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16854 |
| NCBI GenInfo Identifier: | 23503050 |
| NCBI Gene ID: | 1716 |
| NCBI Accession: | Q16854.2 |
| UniProt Secondary Accession: | Q16854,P78532, Q16759, Q4ZG09, Q7L1W9, Q96BC1, |
| UniProt Related Accession: | Q16854 |
| Molecular Weight: | 277 |
| NCBI Full Name: | Deoxyguanosine kinase, mitochondrial |
| NCBI Synonym Full Names: | deoxyguanosine kinase |
| NCBI Official Symbol: | DGUOKÂ Â |
| NCBI Official Synonym Symbols: | dGK; MTDPS3Â Â |
| NCBI Protein Information: | deoxyguanosine kinase, mitochondrial |
| UniProt Protein Name: | Deoxyguanosine kinase, mitochondrial |
| UniProt Gene Name: | DGUOKÂ Â |
| UniProt Entry Name: | DGUOK_HUMAN |
