The DGUOK Antibody (CAB6898) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reacts with human samples, making it ideal for use in Western blot applications. By binding to the DGUOK protein, this antibody allows for the precise detection and analysis of DGUOK in a variety of cell types.DGUOK is essential for maintaining mitochondrial DNA integrity and mutations in the DGUOK gene have been linked to mitochondrial DNA depletion syndromes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DGUOK Antibody
SKU:
CAB6898
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
dGK, NCPH, NCPH1, PEOB4, MTDPS3, DGUOK
Positive Sample:
Jurkat, HepG2, Mouse heart, Mouse kidney, Rat liver, Rat brain
Cellular Localization:
Mitochondrion.
Calculated MW:
32kDa
Observed MW:
40kDa
In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene.
Purification Method
Affinity purification
Gene ID
1716
RRID
AB_2767457
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DGUOK Rabbit pAb (CAB6898) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
