The DHX58 Antibody (CAB8257) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in a variety of applications, including Western blotting. By binding to DHX58, this antibody enables researchers to detect and analyze the protein in different cell types, making it ideal for studies in virology and RNA biology.DHX58, also known as RIG-I-like receptor 1 (RLR-1), plays a crucial role in recognizing viral RNA and initiating immune responses to combat viral infections.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DHX58 Antibody
SKU:
CAB8257
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LGP2, RLR-3, D11LGP2, D11lgp2e, DHX58
Positive Sample:
Mouse spleen, Rat spleen, Rat lung
Cellular Localization:
Cytoplasm.
Calculated MW:
77kDa
Observed MW:
77kDa
Enables double-stranded RNA binding activity; single-stranded RNA binding activity; and zinc ion binding activity. Involved in negative regulation of defense response and negative regulation of type I interferon production. Predicted to be active in cytoplasm.
Purification Method
Affinity purification
Gene ID
79132
RRID
AB_2769166
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using DHX58 Rabbit pAb (CAB8257) at 1:9000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using DHX58 Rabbit pAb (CAB8257) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
