The DIAPH1 Monoclonal Antibody (CAB11596) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows strong reactivity with human samples and has been validated for use in Western blot applications. By binding to the DIAPH1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for research in cell biology, cancer biology, and developmental biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DIAPH1 Monoclonal Antibody
SKU:
CAB11596
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0639
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1729
RRID
AB_2861604
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DIAPH1 Rabbit mAb (CAB11596) at 1：1000 dilution incubated overnight at 4℃.. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Western blot analysis of lysates from 293T cells using DIAPH1 Rabbit mAb (CAB11596) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
